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KMID : 0357119810030010049
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Korean Journal of Immunology
1981 Volume.3 No. 1 p.49 ~ p.61
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Studies of HLA (Human Leucocyte Antigen) Typing in the Korean
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이종훈/Lee, Chong Hoon
이연태/김금용/임병욱/한훈/이용각/민병석/Lee, Yun Tai/Kim, Gum Ryong/Lim, Byung Uk/Hahn, Hoon/Lee, Yong Kak/Min, Byong Sok
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Abstract
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To determine the frequency of HLA-A,B,C and-HLA DR antigens and genes in 200.Korean population, the present study was performed by Terasaki´s Tissue typing method with. the purified T- and B-lymphocytes obtained from 143 normal healthy persons and 57 chronic glomerulonephritis patients during for 16 months from July 1, 1980 to October 31, 1981.
Lymphocytes were isolated from heparinized whole blood by centrifugation over Ficoll/ Hypaque density gradient solution. Contaminated platelets and granulocytes were removed by treatment with thrombin (100 USA N.I.H. units/ml). T- and B-lymphocytes were separated by elution of crudely isolated lymphocytes through the nylon fiber column which was loosenly packedinto´´the long slender transparent plastic tube and eluted with Hank´s balanced salt solution (HBSS). After washing with HBSS, lymphocytes resuspended finally in McCoy´s media were adjust to 1.5 x 106 cells/mL.
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